Fig. 4.

M. bovis BCG is able to recycle PG. a M. bovis BCG WT, ∆lpqI, ∆lpqI::lpqI and ∆lpqI::Empty Vector were incubated with 1 mM 4MU-GlcNAc in minimal media. After 3 days the fluorescence of the cultures were measured (n = 3; error bars are ± SD). b M. bovis BCG WT (grey diamonds) and ∆lpqI (red triangles) were simultaneously evaluated for release of cell wall peptides and growth (n = 3; error bars are ± SD). c M. bovis BCG WT (dark grey bars), ∆lpqI (red bars), and ∆lpqI::lpqI (light grey bars) were evaluated for their growth using MurNAc (5 mM), GlcNAc-MurNAc (2 mM), soluble PG from M. smegmatis mc²155 wild-type or ∆namH (5 and 2.5 mM, respectively), and soluble PG, which had been pre-digested wth LpqI (striped bars) as sole carbon sources using a resazurin assay (n = 3; *** = p < 0.001; ** = p < 0.005). Statistical significance determined using a two-tailed t-test in GraphPad Prism 7