Fig. 5.

Loss of LpqI leads to lysozyme and antibiotic resistance. a–e M. bovis BCG WT (blue), ∆lpqI (red), and ∆lpqI::lpqI (grey) were incubated with increasing concentrations of lysozyme or antibiotics at a starting OD₆₀₀ of 0.1. After 7 days incubation total growth was assessed using a resazurin assay, where total fluorescence correlates with respiration and growth (n = 3; error bars are ± SD; Amox./Clav—amoxicillin plus clavulanic acid; Chlor.—chloramphenicol). f M. bovis BCG WT, ∆lpqI and ∆lpqI::lpqI and ∆lpqI::EV were incubated with EtBr and the rate of EtBr uptake was monitored as an increase in fluorescence. No significant differences were found in pairwise t tests across all strains (n = 3; error bars are ± SD). Statistical significance determined using a two-tailed t test