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. 2019 Jun 14;2:208. doi: 10.1038/s42003-019-0461-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — C/EBPβ-LAP and -LIP isoforms regulate cellular metabolism. a Immunoblot analysis of C/EBPβ-LAP and C/EBPβ-LIP expression in mouse embryonic fibroblasts (MEFs) derived from wt or C/EBPβ^ΔuORF mice. α-tubulin is used for loading control. Uncropped images are shown in Supplementary Fig. 7. b Extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) of MEFs derived from wt or C/EBPβ^ΔuORF mice (n = 6). c Immunoblot analysis of C/EBPβ-LAP and C/EBPβ-LIP expression in wt MEFs and with ectopic expression of LIP. α-tubulin is used for loading control. Uncropped images are shown in Supplementary Fig. 7. d ECAR and OCR of wt MEFs with control empty vector (EV) or ectopic expression of LIP (n = 6). e Immunoblot analysis of C/EBPβ-LAP and C/EBPβ-LIP expression in Cebpb-knockout (ko) MEFs with control empty vector (EV) or ectopic expression of LIP or LAP. β-actin is used for loading control. Uncropped images are shown in Supplementary Fig. 7. f ECAR and OCR of Cebpb-ko MEFs with control empty vector (EV) or ectopic expression of LIP or LAP (n = 6). g Mitochondrial/nuclear DNA ratio and h MitoTracker quantification of Cebpb-ko MEFs with control empty vector (EV) or ectopic expression of LIP or LAP (n = 7 for EV, n = 6 for LIP and n = 5 for LAP). Statistical differences were analysed by Student’s t-tests. Error bars represent SD, *P < 0.05, **P < 0.01, ***P < 0. 001