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. 2019 Jun 17;9:8661. doi: 10.1038/s41598-019-44935-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Hybrid photoacoustic ultrasonic (PAUS) imaging of a porcine brain. The dashed white line and boxes show corresponding sections of the swine cortex. (a) Photograph of a sagittal surgical slice segmented from the extracted brain, 1 cm from the midline. The shown segment is manually registered to (b) a representative photoacoustic (PA) image with two regions of interest (ROI), and (c) the corresponding ultrasound (US) B-Mode image. Both the US and the PA image are shown with in arbitrary units with logarithmic compression. (d) Photograph of the exposed cortex after the craniotomy and dura mater retraction with the dashed line marking the PAUS imaging plane. The electrocorticography (ECoG) electrodes are positioned in the lateral margins. The location of the KCl stimulation is denoted as well.