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. 2019 Jun 17;10:2675. doi: 10.1038/s41467-019-10590-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — The pMMO structure and location of the metal centers. In the crystal structure of Methylocystis sp. strain Rockwell-pMMO (4PHZ), there is a monocopper center in PmoB coordinated by residues His 29, His 133, and His 135 (Cu_B), and a monocopper center in PmoC coordinated by Asp 129, His 133, and His 146 (Cu_C). PmoA, PmoB, and PmoC are shown in pink, purple, and blue, respectively, with one of the three protomers highlighted. Copper ions are shown as yellow spheres