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. 2019 Jun 17;10(7):477. doi: 10.1038/s41419-019-1703-1

Fig. 2. Cav-1 upregulates Pofut1 by increasing transcription factor phosphorylation.

Fig. 2

a Luciferase activity in Hepa1-6, Hepa1-6/Cav-1, Hca-F and Cav-1-knockout Hca-F cells was assayed at 48 h after transfection. Experiments were performed in triplicate, and the statistical significance of the difference was determined using the t-test (**P < 0.01 vs. control). b Schematic representation of predicted binding sites for HNF4A, c-Myc, CREB and Sp1. c ChIP assays confirmed that HNF4A, c-Myc, CREB, and Sp1 can bind to the following regions: region 1 (from −1088 to −1222 bp), region 2 (from −939 to −1045 bp), region 3 (from −483 to −587 bp), and region 4 (from −56 to −197 bp). d A western blot assay was used to detect the phosphorylation levels of HNF4A, Sp1, c-Myc and CREB after overexpression of Cav-1 in Hepa1-6 cells and after knockdown of Cav-1 in Hca-F cells. GAPDH served as a loading control. Statistical comparison using the t-test: **P < 0.01, **P < 0.001