Fig. 5. Caveolin-1 (Cav-1) activates the Notch signaling pathway via Pofut1.

a Western blot analysis of Pofut1, Notch receptor intracellular domain (NICD), HES1, and HEY1 in Hepa1-6, Hepa1-6/Cav-1, Hca-F, and Hca-F-knockout Cav-1 cells. GAPDH served as a loading control. b Immunofluorescence staining was performed to detect the protein expression of Pofut1, NICD, HES1, and HEY1 after overexpression of Cav-1 in Hepa1-6 cells and after knockdown of Cav-1 in Hca-F cells. Nuclei were counterstained with DAPI (×40). c Expression levels of NICD, HEY1, and HES1 were examined by immunohistochemistry staining. The blue staining represents the nucleus, and brown staining represents Cav-1 and Pofut1 (the secondary antibody was labeled with horseradish peroxidase, which produces a brown color with the substrate DAB). Representative sections of liver tissues from wild-type C57BL/6J and Cav-1^−/− C57BL/6J mice. Original magnification, ×100. Statistical comparison using t test: **P < 0.01