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. 2019 Jun 17;9:8674. doi: 10.1038/s41598-019-45203-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Impact of Aza treatment on FCGRT expression. (a) FCGRT mRNA expression in AC16, Hep G2, and SNU-475 cells after Aza treatment (5 µM, 72 h). Plots depict fold differences in FCGRT mRNA expression levels relative to controls (0 µM Aza). Each point represents individual measurements. Horizontal bars show the mean ± SD from three experiments performed in quintuplicates. *** P < 0.001, ns = not significant, Student’s t test. (b) Cellular FcRn protein expression (yellow) before and after Aza treatment (5 µM, 72 h. Representative images). Nuclei were stained with DAPI (blue). Scale bar: 10 µm. (c) Quantitation of FcRn relative fold expression after Aza treatments. Each point represents individual measurements. Horizontal bars show the mean ± SD from two independent experiments (8–10 fields per condition). *** P < 0.001, ns = not significant, Student’s t test.