Table 1.
CryoEM data collection, refinement and validation statistics
| Map 1 (EMDB-4543) (PDB 6QG0) |
Map A (EMDB-4545) (PDB 6QG2) |
|
|---|---|---|
| Data collection and processing | ||
| Magnification | 104,478 | 104,478 |
| Voltage (kV) | 300 | 300 |
| Electron exposure (e−/Å2) | 45 | 21 |
| Defocus range (μm) | 1.5–4.5 | 1.5–4.5 |
| Pixel size (Å) | 1.34 | 1.34 |
| Symmetry imposed | C2 | C1 |
| Initial particle images (no.) | 173,740 | 633,220 |
| Final particle images (no.) | 131,663 | 119,037 |
| Map resolution (Å) | 4.2 | 4.6 |
| FSC threshold | 0.143 | 0.143 |
| Map resolution range (Å) | — | — |
| Refinement | ||
| Initial model used (PDB code) | 5B04 | 5B04 |
| Model resolution (Å) | 4.2 | 4.6 |
| FSC | 0.45 | 0.42 |
| Model resolution range (Å) | — | — |
| Map sharpening B factor (Å2) | −119 | −100 |
| Model composition | ||
| Non-hydrogen atoms | 36,980 | 38,676 |
| Protein residues | 4,742 | 4,961 |
| Ligands | — | — |
| B factors (Å2) | 356 | — |
| Protein | 356 | — |
| Ligand | — | — |
| R.m.s. deviations | ||
| Bond lengths (Å) | 0.008 | 0.008 |
| Bond angles (°) | 1.24 | 1.258 |
| Validation | ||
| MolProbity score | 2.48 (99th) | 2.50 (98th) |
| Clashscore | 5.3 (100th) | 3.68 (100th) |
| Poor rotamers (%) | 5.9 | 21.2 |
| Ramachandran plot | ||
| Favoured (%) | 87.6 | 83.3 |
| Allowed (%) | 10 | 13.2 |
| Disallowed (%) | 2.4 | 3.5 |