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. Author manuscript; available in PMC: 2019 Jun 17.
Published in final edited form as: Mol Cell Endocrinol. 2015 Apr 2;409:51–58. doi: 10.1016/j.mce.2015.03.023

Figure 3: Effects of inhibition of EP2 and EP4 receptors on H3 histone methylation and demethylation machinery proteins in human endometriotic epithelial cells 12Z and stromal cells 22B.

Figure 3:

(A1-A2) Western blot analysis and densitometry of proteins (B1) hSET1, (B2) HP1a, (B3) SUV39H1, (B4) EZH2, (B5) LSD1, (B6) JMJD2A, and (B7) JMJD3 in 12Z and 22B cells. β-actin protein was measured as an internal control. The 12Z and 22B cells were treated with EP2 and EP4 inhibitors (EP-I) EP2 (AH6809–75 μM) and EP4 (AH23848- μM) for 24h. The experiments were performed as we described in the “Materials and Methods”. *-Control vs. EP2-I/EP4-I, P<0.05. Numerical data are expressed as Mean ± SEM of three (n=3) experiments.