Figure 5: Effects of inhibition of EP2 and EP4 receptors on transcriptional activation and suppression machinery proteins in human endometriotic epithelial cells 12Z and stromal cells 22B.
(A1-A2) Western blot analysis and densitometry of proteins (B1) Pol II, (B2) MeCP2, (B3) MBD1, (B4) CoREST, and (B5) Sin3A in endometriotic epithelial cells 12Z and stromal cells 22B. β-actin protein was measured as an internal control. The 12Z and 22B cells were treated with EP2 and EP4 inhibitors (EP-I) EP2 (AH6809–75 μM) and EP4 (AH23848- μM) for 24h. The experiments were performed as we described in the “Materials and Methods”. *-Control vs. EP2-I/EP4-I, P<0.05. Numerical data are expressed as Mean ± SEM of three (n=3) experiments.