Table 1:
Details of Antibodies used.
| Antibodies | Manufacturer | Cat # | Concentration used in western blot |
|---|---|---|---|
| DNMT1 | Abcam | ab13537 | 1:200 |
| DNMT3a | Abcam | ab2850 | 1:400 |
| DNMT3b | Abcam | ab13604 | 1:200 |
| H3K4me3 | Abcam | ab8580 | 1:1000 |
| H3K9ac | Abcam | ab4441 | 1:500 |
| H3K9me3 | Abcam | ab8898 | 1:1000 |
| H3K27ac | Abcam | ab4729 | 1:1000 |
| H3K27me3 | Abcam | ab6002 | 1:1000 |
| hSET 1 | Abcam | ab70378 | 1:1000 |
| HP1a | Abcam | ab77256 | 1:2000 |
| SUV39H1 | Cell Signaling | 8729 | 1:1000 |
| EZH2 | Abcam | 3748 | 1:200 |
| LSD1 | Cell Signaling | 2139 | 1:1000 |
| JMJD2A | Cell Signaling | 3393 | 1:1000 |
| JMJD3 | Cell Signaling | 3457 | 1:1000 |
| HDAC1 | Cell Signaling | 5356 | 1:1000 |
| HDAC2 | Cell Signaling | 5113 | 1:1000 |
| HDAC3 | Cell Signaling | 3949 | 1:1000 |
| p300 | Santa Cruz | sc-585 | 1:500 |
| Pol II | Santa Cruz | sc-899 | 1:500 |
| MeCP2 | Abcam | ab2828 | 1:1000 |
| MBD1 | Abcam | ab2846 | 1:500 |
| CoREST | Millipore | 07–455 | 1:2000 |
| Sin3A | Abcam | ab3479 | 1:2000 |
| β-actin | Sigma | A2228 | 1:8000 |
| Goat anti-rabbit IgG | Kirkegaard & Perry Laboratories | 474–1506 | 1:10000 |
| Goat anti-mouse IgG | Kirkegaard & Perry Laboratories | 474–1806 | 1:10000 |
| Rabbit anti-goat IgG | Kirkegaard & Perry Laboratories | 14–13-06 | 1:5000 |