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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Nat Cell Biol. 2019 Apr 1;21(4):420–429. doi: 10.1038/s41556-019-0301-x

Figure 2. Precocious hyaloid vessel regression in the Opn5 null and absence of 380 nm photons.

Figure 2.

a, b, Hoechst 33258 (blue) labelled hyaloid vessel preparations from P1 Opn5+/+ and Opn5−/− mice. c, d, Higher magnification images of two examples each of P1 hyaloid vessel segments from Opn5+/+ (c) and Opn5−/− (d) mice labelled with Hoechst 33258 (presented in red) and isolectin (green). e, f, Hoechst 33258 labelled hyaloid vessel preparations from P8 Opn5+/+ and Opn5−/− mice. g, Quantification of hyaloid vessel number in Opn5+/+ (grey), Opn5+/− (light blue) and Opn5−/− (dark blue) mice over a P1-P8 time-course. h, as in (e) but relative hyaloid vessel numbers for control (grey), Opn5−/− (blue), and Opn4−/− (green) mice. i, j, Hoechst 33258 labelled hyaloid vessel preparations from P8 Opn5fl/fl control and Opn5fl/fl; Rx-cre mice. k, Quantification of P8 hyaloid vessel number in Opn5fl/fl control (grey), Opn5fl/fl; Chx10-cre (light blue), and Opn5fl/fl; Rx-cre (dark blue) mice. l, m, Hoechst 33258 labelled hyaloid vessel preparations from P8 mice raised from birth in full spectrum (V, violet + B, blue, + G, green, + R, red) or "minus violet" (BGR) lighting. n, Quantification of P8 hyaloid vessel number from P8 mice raised from birth in full spectrum (grey bar) or “minus violet” (blue bar) lighting. All p-values by Student T-test. The number at the base of each chart is n and represents the number of animals assessed. Error bars are SEM. Scale bars 400 μm (a,b,e,f,I,j,l,m) 20 μm (c,d).