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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Nat Cell Biol. 2019 Apr 1;21(4):420–429. doi: 10.1038/s41556-019-0301-x

Figure 3. OPN5- and light-dependent pathways regulate dopamine levels in the neonatal mouse eye.

Figure 3.

a-f, Immunofluorescent labelling for tyrosine hydroxylase (TH, greyscale) in flat mount retinae from control Opn5+/+ (a, b, e) and Opn5−/− (c, d, f) mice at P8 (a-d) and P15 (e, f). Scale bars 100 μm (a-d) 20 μm (e,f). g-k, Charts showing ELISA quantification of dopamine in retinal lysates (g, j) and vitreous fluid (h, i k). g, h, Dopamine levels in each tissue over a P2-P8 developmental time-course. i, Dopamine levels over a P2-P6 developmental time course comparing normal lighting (LD) with the consequences of dark rearing (DD). j, k, Dopamine levels in retinal lysate (j) and vitreous fluid (k) from P6 Opn5+/+ control (j, k, grey bars), Opn5+/− heterozygote (j, k, light blue bars), and Opn5−/− homozygote (j, k, light blue bars). p-values by (g, h) Two Way ANOVA, (i) Student’s T-test, (j, k) One Way ANOVA. Error bars are SEM. The number at the base of each chart is n and represents the number of animals assessed. Panels a-f are representative of at least 3 separate experiments. Additional examples of (a-d) are available on Figshare.