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. Author manuscript; available in PMC: 2019 Jun 17.
Published in final edited form as: Surgery. 2018 Jun 6;164(3):473–481. doi: 10.1016/j.surg.2018.04.012

Fig. 2.

Fig. 2.

Morphology and viability of spheroids, and differences in transduction efficiency between single-cell and spheroid hepatocytes. (A) Light microscopy image of spheroids. (B) Inverted fluorescence microscopy image of spheroid hepatocytes, using the Fluoroquench viability stain before transplantation in pig 897; live cells (green) and necrotic cells (orange). (C) Ex vivo transduction of primary pig single-cell hepatocytes and hepatocyte spheroids with LV-GFP at MOls of 500 and 2000 LPs per cell and quantification of GFP-positive cells detected by flow cytometry 96 hours after transduction. Data are means ± SD ( n=3 replicates for single cells, 2 replicates for spheroids). *P < .01.