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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Plant Cell Rep. 2018 Dec 14;38(3):311–319. doi: 10.1007/s00299-018-2367-5

Fig. 1.

Fig. 1

The CLV3p signal mediates rapid MAPK activation and WUS suppression in the SAM. a A schematic strategy for studying stem cell signaling using the clv3 SAM. CLV3, CLV1 and WUS genes related to stem cell signaling exhibit different expression domains in the SAM. Overlapped expression of CLV1 and WUS domains in the clv3 SAM show the ready-state for direct response triggered by CLV3p signals. b MAPK phosphorylation by CLV3p in clv3–2 fls2–24 SAMs treated with 1 μM CLV3p for 0 min, 30 min or 60 min. Protein in situ immunohistochemistry was performed using an α-phospho-MAPK antibody. c WUS repression by CLV3p in the same context with (b) through RNA in situ hybridization. Scale bar, 50 μm (b, c).