Expression of WUS and WUS-target genes is impaired in the loss-of-function mpk3,6 mutants. The expression of WUS-specific target genes (a) and WUS (b) in WT, mpk3, mpk6 and mpk3,6-es1/2 seedlings treated with 10 μM estradiol for 5 days. RNAs were isolated from the shoot apex tissues. Relative expression was normalized by ACT2. Error bars are ±S.D. (n=3). Significant differences compared to WT were determined via one-way ANOVA followed by Turkey’s test: * P<0.05, ** P<0.01 and *** P<0.001.