Fig. 10.

a These confocal microscopy and IHC images (i–iv) provide intra-cellular visualizations of SERCA2a within the corresponding experimental groups. SERCA2a is identified in green fluorescence and nuclei are identified by blue fluorescence. b This graph exhibits the fluorescent intensity of SERCA2a 8 weeks after TAC and was provided by the data collected from the Image J software. Each fluorescent intensity corresponds to SERCA2a’s presence within the specific experimental groups. With respect to SERCA2a fluorescent intensity, the TFAM overexpression Sham group was significantly greater relative to the WT Sham group. Also, the TFAM TAC group was shown to be significantly greater in intensity of TFAM relative to the WT TAC group. c This graph displays SERCA2a proteins expression 8 weeks after the aortic banding surgery. Lysates were analyzed by western blotting for SERCA2a protein expression. The TFAM overexpression Sham group was significantly greater in protein expression of SERCA2a relative to the WT Sham group. The TFAM overexpression TAC group was significantly greater in protein expression of SERCA2a relative to the WT TAC group. Both TAC groups were significantly decreased in SERCA2a protein expression when compared to their Sham counterparts. The bands were quantified using densitometry and the data were graphed for an N of 5 using SEM for significance. Values are expressed as ± SEM. N = 5. *P < 0.05, **P < 0.025, ***P < 0.001