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. Author manuscript; available in PMC: 2019 Jun 17.
Published in final edited form as: J Vis Exp. 2018 Nov 4;(141):10.3791/58399. doi: 10.3791/58399

Materials

Name Company Catalog Number Comments
2-(2-nitro-4-trifluoromethylbenzoyl)-1,3-cyclohexanedione (NTBC) Yecuris 20–0027
12 mm Trocar Covidien B12STS
5 mm Trocar Covidien B5SHF
Endo Surgical Stapler 60 Covidien EGIA60AMT
Endo Surgical Stapler 45 Covidien EGIA45AVM
Endo Surgical Stapler 30 Covidien SIG30AVM
Endo catch bag Covidien 173050G
0 PDS Ethicon Z340H
2–0 Vicryl Ethicon J459H
4–0 Vicryl Ethicon J426H
Dermabond Ethicon DNX12 Sterile Dressing
Williams’-E Powder Gibco ME16060P1
NaHCO3 Sigma Aldrich S8875–1KG
HEPES Fisher BP310–1
Pen/Strep Gibco 15140–122
Fetal Bovine Serum Corning 35–011-CV
NaCl (g/L) Sigma Aldrich S1679–1KG
KCl (g/L) Sigma Aldrich P3911–500G
EGTA (g/L) Oakwood Chemical 45172
N-acetyl-L-cysteine Oakwood Chemical 3631
(N-A-C, g/L) Sigma Aldrich A9165–100G
CaCl2 2H2O (g/L) Sigma Aldrich 223506–500G
Collagenase D (mg/mL) Crescent Chemical 17456.2
Dulbecco's modified eagle medium Corning 15–013-CV
(DMEM)
Dexamethasone Fresenius Kabi NDC6337
Epidermal Growth Factor Gibco PHG0314