Fig. 4.

Binding site overlap of Mediator complex and its interactors. a Percentage overlap of genome-wide binding sites of Mediator (Med1) with Mediator-interactors Tcf4, Sox2, NFI (Nfia + Nfib), Carm1, Jmjd1c, Chd7, Ep300, Integrator complex (Ints11 subunit), and RNApol2 in NSCs. Cbx8 and Ctcf were not identified as Mediator interactors and serve as negative controls. Percentages overlap of binding sites, as determined by ChIP-seq, are indicated. T + S + N, sum of the binding sites of Tcf4, Sox2, and NFI. TSS, within 1 kb of a transcription start site. b Overlap of binding sites of Mediator (Med1) with binding sites of Mediator interactors at the Myc locus in NSCs. ChIP-seq tracks for the indicated proteins and histone modifications at the Myc gene are shown. The Myc SE in the adjacent (inactive) Pvt gene is indicated with a red bar. Range of reads per million per base pair is indicated on the y-axis. Scale bar is indicated. c Overlap of binding sites of Mediator (Med1) with binding sites of Mediator interactors at the Rad51c locus in NSCs. ChIP-seq tracks for the indicated proteins and histone modifications at the Rad51c gene are shown. The Rad51c SEs in the adjacent (inactive) Tex10 gene are indicated with red bars. Range of reads per million per base pair is indicated on the y-axis. Scale bar is indicated. d Overlap of genome-wide binding sites of Mediator interactors and Cbx8 and Ctcf in NSCs. Percentages overlap of binding sites, as determined by ChIP-seq, are indicated