Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jun 17;10:2669. doi: 10.1038/s41467-019-10502-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — Mediator genome recruitment upon knock-down of Tcf4 or Sox2. a Relative mRNA levels of Tcf4 48 h after transfection with pSuper-Tcf4-shRNA or pSuper-control-shRNA (left panel), Relative mRNA levels of Sox2 46 h after transfection with pSuper-Sox2-shRNA or pSuper-control-shRNA (right panel). Source data are provided as a Source Data file. b Mediator ChIP signal on selected enhancers at the indicated distances from the TSS of the indicated genes. RT-PCR signals on the indicated genome areas of Med12 ChIP (Med12) and control rabbit IgG ChIP (IgG) are indicated as percentage of chromatin input. Amylase (Amy) functions as a negative control genomic region. S.e.m. is indicated of two independent experiments. Source data are provided as a Source Data file. c Mediator ChIP signal at selected enhancers upon knock-down of Tcf4. Med12 ChIP RT-PCR signals on the indicated genome areas in NSCs transfected with a plasmid expressing Tcf4-shRNA are indicated as percentage of the ChIP signal of NSCs transfected with a plasmid expressing control shRNA. S.e.m. is indicated of two independent experiments. Significance of the difference in Med12 ChIP signal between Tcf4-depleted NSCs and control NSCs was assessed by an unpaired Student t-test (*p < 0.05, **p < 0.01). Source data are provided as a Source Data file. d Mediator ChIP signal at selected enhancers upon knock-down of Sox2. Med12 ChIP RT-PCR signals on the indicated genome areas in NSCs transfected with a plasmid expressing Sox2-shRNA are indicated as percentage of the ChIP signal of NSCs transfected with a plasmid expressing control shRNA. S.e.m. is indicated of two independent experiments. Significance of the difference in Med12 ChIP signal between Sox2-depleted NSCs and control NSCs was assessed by an unpaired Student t-test (*p < 0.05, **p < 0.01). Source data are provided as a Source Data file