Fig. 4.

Glial and ECM boundaries between clusters of ectopic Purkinje cell masses in early postnatal scm/scmand rl/rl cerebella. A, Double immunofluorescence of flattened tangential sections reveals islands of rhodamine-labeled, calbindin-positive Purkinje cells (P, rather dim in this black and white image) separated by areas of intense fluorescein tenascin labeling (arrowheads delineate border between Purkinje cells and tenascin boundary). B, Double immunofluorescence of a coronal section reveals a midline GFAP-positive glial raphe (rhodamine label with dimmer appearance,arrow) between two calbindin-positive Purkinje cell masses (P, bright-appearing fluorescein label). C, GFAP-positive immunofluorescence at the midline in the reeler cerebellum (arrow).Asterisks mark the pial surface. Scale bar, 100 μm.