Fig. 6.

Effects of K252a and staurosporine on trkB.T1 and trkB.T2 signaling capability. A, TrkB.T1 transfected cell lines were pretreated for 20 min with either no inhibitor, 100 nm K252a, or 200 nm staurosporine and then treated simultaneously with the same inhibitor plus 50 ng/ml BDNF at time t = 0 and analyzed as in Figure 5.B, TrkB.T2 transfected cell lines were pretreated for 20 min with either no inhibitor, 100 nm K252a, or 200 nm staurosporine and then treated simultaneously with the same inhibitor plus 50 ng/ml BDNF at time t = 0 and analyzed as in Figure 5. All exposures to BDNF or BDNF plus inhibitor were for the duration of the experiment. For both the staurosporine and K252a experiments, these are relatively high concentrations that likely inhibit multiple kinases.