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. 1997 Feb 1;17(3):1112–1121. doi: 10.1523/JNEUROSCI.17-03-01112.1997

Fig. 2.

Fig. 2.

A, A representative RPA of total RNA (20 μg/lane) from saline- and cocaine-treated day 60 fetal monkey brain tissues illustrating the levels of PPD mRNA detected in the different brain regions of individual animals. UP, undigested probe; DP, digested probe; S, saline-treated; C, cocaine-treated.A1–A6 represent defined brain regions (see Materials and Methods). B, Linear regression analysis of the optical densities of the PPD mRNA sense standard curve revealedr = 0.995. C, Distribution and quantitative analysis of PPD mRNA in brain tissue obtained from saline-treated and cocaine-treated fetal macaques (n = 3 each). Densitometric scannings were normalized to cyclophilin mRNA and quantified from each sense mRNA standard curve. PPD mRNA was significantly increased in A1 (FC/ST) in cocaine-treated animals (*p < 0.05; paired two-tailed Student’st test).