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Aβ induces HNE production and conjugation to the glucose transporter. Neocortical cultures were exposed for 4 hr to 10 μm Aβ25-35 (A), 10 μm HNE (H), or vehicle (V). Solubilized total cell protein was immunoprecipitated with an antibody against the glucose transporter, and the antibody-bound proteins were separated by SDS-PAGE, transferred to nitrocellulose, and immunoreacted with an HNE antibody (see Materials and Methods).
