Fig. 4.

NFHLacZ inclusions in neuronal perikarya are more resistant to trypsin digestion than native NFs in axons of the corpus callosum in control mice. A and Billustrate the corpus callosum of a wild-type (WT) mouse, whereas C andD show NF inclusions in the neocortex of a NFHLacZ (TG) mouse. The sections in A andC were stained with the mAb RMO189 before trypsin treatment (TS−), whereas the adjacent sections inB and D were stained with the same mAb after trypsin treatment (TS+). Although immunoreactive NFM in normal axons of the corpus callosum is completely eliminated by trypsin digestion in the wild-type mouse, the same does not eliminate NFM immunoreactivity in the NFHLacZ inclusions of the NFHLacZ transgenic mouse. The sections in A–Dwere counterstained with hematoxylin.A–D are at the same magnification; scale bar: D, 50 μm.