Table 2.

List of antibodies for immunohistochemistry

Antibody	Dilution	Epitope	Inclusion staining
Rabbit anti-β-Gal	200×	NA	++
Rabbit Anti-NFL	2000×	NFL, Pind/tail	++
RM03	Neat	NFM, Pind/tail outside MPR	++
RM0189	100×	NFM, Pind/core	++
RM0255	200×	NFM, Pind/tail, last 20 amino acids	++
RM032	50×	NFM, P+++/tail within MPR	++
RM024	10×	NFH, P+++/tail within MPR	+
RM0217	10×	NFH, P++/tail within MPR	+
TA51	2000×	NFH, P+/tail within MPR	++
RMd09	1000×	NFH, P−/tail within MPR	++
Actin	100×	NA	−
α-Tubulin	10,000×	NA	−
β-Tubulin	10,000×	NA	−
GFAP	100×	NA	−
Ubiquitin	20,000×	NA	Variable
MAP2	Neat	NA	−
MAP5	Neat	NA	−
Anti-synaptophysin	50×	NA	−

P+++, Heavily phosphorylated epitope in NFM or NFH; P++, moderately phosphorylated epitope in NFM or NFH; P+, lightly phosphorylated NFM or NFH epitope. P−, non- or poorly phosphorylated epitope in NFM or NFH; Pind, phosphorylation-independent epitope in NFM; MPR, multiphosphorylation repeat in NFM or NFH; NA, not applicable.