Table 1.

hDAT turnover rate determined from saturation analyses of DA uptake velocity and [³H]mazindol binding to intact oocytes

DA uptake1-a		[3H]mazindol binding		Turnover rate
Vmax	KT	Bmax	KD	Vmax/Bmax
(fmol/(oocyte × sec))	(μm)	(fmol/oocyte)	(nm)	(sec−1)
66.9 ± 6.3	2.71-b	152.5 ± 7.0	5.21-c	0.47 ± 0.06

Mean values (±SEM) for V_max andB_max were determined in three experiments using different oocyte batches (not voltage-clamped). In each experiment, parallel pools of oocytes were used for V_max and for B_max measurements.

^F1-aDA uptake was measured solely with [³H]DA in one experiment and solely by HPLC-EC in a second experiment. Both methods were used in a third experiment and yielded V_max values that differed by 7%.

^F1-bCI₉₅ 0.2–36 μm.

^F1-cCI₉₅ 2.9–9.2 nm.