Fig. 5.

Shh supports the survival of midbrain GABA⁺ neurons. A, In addition to supporting the survival of TH⁺ cells in the midbrain cultures, Shh promotes the survival of GABA-ir neurons with a similar dose– response (error bars represent SEM) (for TH,p < 0.001 at 25 and 50 ng/ml; for GABA,p < 0.001 at 25 and 50 ng/ml). B, Double-label immunofluorescence of Shh-treated cultures shows that the majority of the GABA+ cells (orange) do not overlap with the TH⁺ cells (green). Scale bar, 15 μm.