Fig. 3.

“Polyp-like” hyperplasia in the SVZ of EGF-treated animals at the end of treatment (2 weeks).A, High density of BrdU-positive cells at the convex pole of a hyperplasia, which protrudes into the CSF-filled ventricle.B, BrdU-positive cells are immunonegative for neuronal (NeuN, red) and astrocytic markers (S100β,blue). The ependymal layer (S100β,blue) is discontinuous (arrows) in areas of growth. C, Density of BrdU-labeled cells is still increased; however, the hyperplastic changes completely regress 4 weeks after EGF withdrawal. Scale bars in A, C, 25 μm.