Fig. 6.

Reduced rostral migration of BrdU-positive cells at the end of EGF and FGF-2 infusions. Compared with aCSF controls (A), the number of BrdU-positive cells (green) in the RMS is decreased in FGF-2 (B) and decreased further in EGF-treated animals (C). However, PSA-NCAM (red), which is required for migration of progenitors within the RMS, is present in all groups. No BrdU-positive cells can be found at 4 weeks after infusion (D–F). Images D–F are immunofluorescent double labelings for BrdU (green) and S100β (blue). Note that scanning parameters for the confocal microscope are identical for A–F. Scale bar in A, 50 μm.