Fig. 7.

Cellular phenotype of newborn cells in the olfactory bulb. Cells in the olfactory granule cell layer were characterized at 4 weeks after infusion of aCSF (A), FGF-2 (B), or EGF (C) for BrdU (green), NeuN (red), and S100β (blue). After aCSF or FGF-2 treatment the vast majority of newborn cells double labels for NeuN (green/red). Note the reduced number of BrdU-positive cells that reach the olfactory bulb in EGF-treated animals (C; see also Table 1). The differentiation of EGF-induced progenitors was shifted toward a glial lineage. Arrows indicate newborn astrocytes (green/blue). Scale bar inA, 20 μm.