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Cellular phenotype of newborn cells in the dentate gyrus. Cells in the hippocampal granule cell layer were characterized at 4 weeks after infusion of aCSF (A), FGF-2 (B), or EGF (C) for BrdU (green), NeuN (red), and S100β (blue). After aCSF or FGF-2 treatment the vast majority of newborn cells double labels for NeuN (green/red). The differentiation of EGF-induced progenitors has shifted toward a glial lineage (C). Arrow indicates a newborn astrocyte (green/blue). Scale bar in A, 20 μm.
