Table 1.
Density and cell fate of newborn cells in the subventricular zone, olfactory bulb, and striatum
| Area | aCSF | FGF-2 | EGF |
|---|---|---|---|
| Subventricular zone | |||
| 1 d after infusion | |||
| Cannula side | 62.2 ± 9.8 | 208.5 ± 62.21-160 | 595.5 ± 133.11-160 |
| Contralateral side | 37.1 ± 8.1 | 32.6 ± 4.4 | 70.3 ± 9.91-160 |
| 4 weeks after infusion | |||
| Cannula side | 30.6 ± 10.7 | 104.8 ± 27.71-160 | 153.6 ± 27.81-160 |
| Contralateral side | 13.3 ± 3.9 | 13.5 ± 2.7 | 88.0 ± 11.81-160 |
| Rostral migratory str. | |||
| 1 d after infusion | 915.5 ± 50.0 | 714.4 ± 38.0* | 368.8 ± 25.01-160 |
| Olfactory bulb | |||
| 4 weeks after infusion | |||
| Total | 40.9 ± 2.0 (100) | 46.9 ± 1.2* (100) | 16.3 ± 1.61-160 (100) |
| Neurons | 39.3 ± 1.6 (96) | 45.2 ± 0.9* (96) | 11.8 ± 1.51-160 (72) |
| Astrocytes | 0.16 ± 0.11 (<0.1) | 0.26 ± 0.10 (<0.1) | 2.26 ± 0.541-160 (14) |
| Striatum | |||
| 4 weeks after infusion | |||
| Total | 2.8 ± 0.4 (100) | 6.1 ± 0.81-160 (100) | 12.3 ± 3.51-160 (100) |
| Neurons | 0 (0) | 0 (0) | 0 (0) |
| Astrocytes | 0.2 ± 0.1 (6) | 1.4 ± 0.41-160 (22) | 4.5 ± 1.41-160 (39) |
| BrdU+/satellite cells | 1.1 ± 0.1 (40) | 3.2 ± 0.5* (53) | 5.2 ± 1.1* (46) |
| BrdU+/sat./astrocytes | 0.05 ± 0.03 (1.8) | 0.6 ± 0.2* (10) | 1.7 ± 0.7* (14) |
The brain areas were selected for unbiased quantification, as shown in Figure 1. Densities of newborn cells after aCSF, FGF-2, and EGF infusion are presented as the mean number of BrdU-positive cells (in thousands) per mm3 ± SEM.
*
p < 0.05;
F1-160: p < 0.01. To determine the cell type of BrdU-positive cells 4 weeks after infusion, we used NeuN as a marker for neurons and S100β for astrocytes. Percentages of cell types (numbers in parentheses) are based on the total density of BrdU-positive cells.