Table 2.

Density and cell fate of newborn cells in the dentate gyrus and cerebellum

Area	aCSF	FGF-2	EGF
Dentate gyrus
1 d after infusion
Granule cell layer	5.60  ± 0.49	4.87  ± 1.06	7.77  ± 1.10
Hilus	1.09  ± 0.34	1.14  ± 0.22	2.06  ± 0.59
Molecular layer	0.66  ± 0.11	0.92  ± 0.17	3.69  ± 0.322-160
4 weeks after infusion
Granule cell layer
Total	3.44  ± 0.56  (100)	3.25  ± 0.32  (100)	2.84  ± 0.89  (100)
Neurons	3.19  ± 0.52  (93)	2.91  ± 0.27  (90)	1.50  ± 0.522-160  (53)
Astrocytes	0.01  ± 0.01  (0.2)	0.01  ± 0.01  (0.2)	1.13  ± 0.352-160  (40)
Hilus	0.57  ± 0.17	0.67  ± 0.14	1.02  ± 0.22
Molecular layer	0.62  ± 0.07	0.70  ± 0.10	2.32  ± 0.472-160
Cerebellum
1 d after infusion
Molecular layer	0.081  ± 0.019	0.160  ± 0.035	0.133  ± 0.027
Granule cell layer	0.065  ± 0.027	0.113  ± 0.007	0.065  ± 0.025
White matter	0.084  ± 0.041	0.050  ± 0.031	0.082  ± 0.018
4 weeks after infusion
Molecular layer	0.069  ± 0.008	0.073  ± 0.016	0.094  ± 0.009
Granule cell layer	0.079  ± 0.007	0.084  ± 0.021	0.123  ± 0.017
White matter	0.079  ± 0.012	0.095  ± 0.028	0.152  ± 0.027

The brain areas were quantified by unbiased sampling methods. Densities of newborn cells after aCSF, FGF-2, and EGF infusion are presented as the mean number of BrdU-positive cells (in thousands) per mm³ ± SEM.

F2-160: p < 0.01. To determine the cell type of BrdU-positive cells in the dentate gyrus 4 weeks after infusion, we used NeuN as a marker for neurons and S100β for astrocytes. Percentages of cell types (numbers in parentheses) are based on the total density of BrdU-positive cells.