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. 1997 Aug 1;17(15):5687–5696. doi: 10.1523/JNEUROSCI.17-15-05687.1997

Fig. 1.

Fig. 1.

Specificity of GKAP antibodies and primary structure of GKAPL N-terminal splice variant.A, Two independent GKAP antibodies recognize recombinantly expressed GKAP and identical 95 and 130 kDa bands in rat brain membranes. COS-7 cells transfected with GKAP cDNA, and rat cortical membranes (10 μg protein) were immunoblotted with GKAP antibodies N1564 and C9589, as indicated. Heterologously expressed GKAP comigrates with the 95 kDa immunoreactive brain protein. Ctx, Cortical membranes. B, Comparison of heterologously expressed GKAP, GKAPL, and rat hippocampal membranes, immunoblotted with N1564 antibodies. Heterologously expressed GKAPL comigrates with the ∼130 kDa immunoreactive brain protein. The same result was obtained with C9589 antibodies (data not shown). Hpc, Hippocampal membranes.C, Amino acid sequence alignment comparing the N-terminal GKAP splice variant GKAPL to GKAP.Splice, Presumed alternative splice site.