Fig. 4.

Responses to alphaxalone of cells expressing mutated β2 subunits. Each panel shows traces recorded from a cell exposed to 10 μm alphaxalone (dotted trace) and then to 10 μm alphaxalone plus 1 mm of an antagonist (solid trace). Actions of 1 mm gabazine are shown in the top rowand of 1 mm bicuculline in the bottom row. Cells transfected with α1β2(Y157S)γ2L subunits (left) showed potentiation between alphaxalone and either gabazine or bicuculline. Cells transfected with α1β2(Y205S)γ2L subunits showed block by either compound, but the block produced by bicuculline was reduced over that seen with wild-type receptors, whereas the block produced by gabazine was increased (see Figs. 3, 6). Calibration in each panel: 20 pA, 10 sec.