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. 1997 Sep 15;17(18):7166–7179. doi: 10.1523/JNEUROSCI.17-18-07166.1997

Fig. 3.

Fig. 3.

Effects of graded levels of prostaglandin synthesis blockade on IL-1-stimulated Fos-ir and CRF mRNA expression. Bright-field photomicrographs show sections through similar levels of the PVH (top) and rostral ventrolateral medulla (bottom) stained for Fos-ir, and dark-field photomicrographs show PVH sections hybridized with probes for CRF mRNA (middle) from animals pretreated intravenously with either vehicle of varying doses of indomethacin (Indo) 15 min before an intravenous challenge with 1.87 μg/kg IL-1β. Indomethacin pretreatment produces a dose-related decrease in the expression of Fos-ir and CRF mRNA in the PVH and of Fos-ir in the region of the C1 catecholamine cell group. The patterns and strength of expression of both markers in the PVH and of Fos-ir in the VLM at the higher indomethacin doses are not distinguishable from those seen in rats injected with vehicle in lieu of IL-1. Note that Fos-ir expression in the regions immediately adjoining the PVH is ostensibly unaffected by any dosage of indomethacin. Magnification, 60×.