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. 1997 Sep 15;17(18):7007–7016. doi: 10.1523/JNEUROSCI.17-18-07007.1997

Fig. 1.

Fig. 1.

Top. Anti-pY490 detects activated TrkA, TrkB, and TrkC. TrkA-, TrkB-, and TrkC-expressing NIH3T3 cells were stimulated with neurotrophins and immunostained with the pY490 antibody, followed by avidin–biotin–HRP and DAB. TrkA, TrkB, and TrkC 3T3 cells stimulated with neurotrophin show increased pY490 immunostaining (+), as compared with control cells stimulated with vehicle alone (−). Immunostaining is abolished by preincubation of the antibody with its phosphopeptide immunogen (phosphopeptide). Preincubation of the antibody with the corresponding unphosphorylated peptide (peptide) or a phosphopeptide corresponding to the NPXY motif of the erbB2 receptor (erbB2 phosphopeptide) does not abolish the staining. No staining is observed in untransfected NIH3T3 cells (NIH). Scale bar, 10 μm.