Fig. 3.

Autoradiograph of CSAD in highly purified preparations. Purified CSAD obtained as described (Tang et al., 1996) was phosphorylated using conditions similar to those described (Bao et al., 1994, 1995), except that exogenous kinase and CIP were included as indicated. Lane 1: control in PKC buffer; lane 2: PKC treatment; lane 3: PKC + CIP; lane 4: staurosporine + PKC; lane 5: PKC inhibitory peptide + PKC; lane 6: PKA; lane 7: control in PKA buffer; lane 8: Coomassie blue staining of the purified CSAD used for the above phosphorylation experiments.