Table 1.

Effect of kinase and phosphatase on CSAD activity in the purified preparation

Treatment	CSAD activity (% of control)
PKC control	100  ± 5
PKC treatment	352  ± 14
PKC + staurosporine	107  ± 6
PKC + CIP	102  ± 4
CIP control	100  ± 5
CIP treatment	6  ± 2
PKA control	100  ± 5
PKA treatment	85  ± 6

Purified CSAD was treated under the following conditions. For the PKA control, the same incubation conditions were used as in the group treated with PKA, except that ATP and the catalytic subunit of PKA were omitted. For the phosphatase (CIP) control, the conditions were the same as those for the group treated with CIP, except that no CIP was included. CSAD activity was determined by the radiometric method, as described (Wu, 1982). Each value is the mean ± SE (n = 4).