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FGF posteriorizes anterior neural tissue induced by DN-BR or chordin. RNA encoding DN-BR (1 ng) or chordin (1.5 ng) was injected into the animal pole area of the two-cell-stage embryos. ACs were dissected, treated with FGF, and processed as described in Figure2. Expression of NCAM, Otx2,Krox20, HoxB9, actin, andEF-1α was tested by RT-PCR.
