Oxidative insults and HNE induce rapid and delayed cell death in PC12 cells: Bcl-2 prevents delayed cell death.A, B, PC12-V (A) and PC12-Bcl2 (B) cells were exposed to the indicated concentrations of HNE, Aβ, or FeSO4 for the indicated time periods, and levels of MTT reduction were quantified (mean and SEM of determinations made in four culture wells/condition).C, D, PC12-V cells (C) and PC12-Bcl2 cells (D) were exposed to vehicle (Control) or the indicated concentrations of HNE, Aβ, orFeSO4, and trypan blue-positive and nonstaining cells were counted. Values are the mean percentage of trypan blue-positive cells in four separate cultures.