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. 1997 Jul 1;17(13):4987–4993. doi: 10.1523/JNEUROSCI.17-13-04987.1997

Fig. 4.

Fig. 4.

Detection of appican in ECM. ECM was prepared from untransfected, KL-APP733-transfected, and KL-APP733S/A-transfected C6 cell cultures labeled with [35SO4 ] for 24 hr. Labeled ECM fractions were extracted and immunoprecipitated with α-5 antibody, and immunoprecipitates were analyzed on 6% SDS-PAGE (see Materials and Methods). Lane 1, ECM fraction from untransfected C6 cells; lane 2, ECM fraction from KL-APP733-transfected C6 cells; lane 3, ECM fraction from KL-APP733-transfected C6 cells treated with chondroitinase ABC; lane 4, ECM fraction from KL-APP733S/A-transfected C6 cells.