Fig. 5.

Attachment assay for primary astrocytes, PC12, N2A, and 293 cells on appican-containing ECM. Attachment experiments were performed as described in Figure 3. Primary astrocytes (A), PC12 (B), N2a (C), and 293 cells (D) were plated on ECM plates prepared from C6 cells transfected with KL-APP733 (hatched bars) or KL-APP₇₃₃S/A (open bars). The incubation time for primary astrocytes, N2a cells, and 293 cells was 30 min, whereas the time for PC12 cells was 2 hr, attributable to the slow attachment displayed by these cells. Percentage of cells attached was calculated as described in Figure 3.