Fig. 2.
Effect of CCK-8S (500 nm) on holding current and whole-cell conductance in a CA1 stratum radiatum interneuron voltage-clamped at −55 mV. A, Time course illustrating the inward change in holding current with CCK-8S and the apparent reversal by the CCKB antagonist PD-135,158 (500 nm). B, Same cell as in A. Effect of CCK-8S on whole-cell steady-state membrane conductance in response to a brief hyperpolarizing voltage step (−10 mV, 300 msec). Note that the inward current caused by CCK-8S was temporally related to the decrease in whole-cell conductance. The delay in response onset was partly attributable to a 1–1.5 min lag time in the bath superfusion system.