Fig. 3.

Localization of PS1 during the initial stages of neuronal differentiation. A, Rat hippocampal neurons after 1 d in culture show PS1 immunoreactivity homogeneously distributed in the cell body and primary neuritic processes. Immunocytochemistry was performed with Aβ 231 to the PS1 N terminus. Scale bar, 10 μm. B, Examination at a higher magnification shows that PS1 is present in neuritic growth cones. Scale bar, 3 μm. C, Negative immunostaining is obtained after preabsorption of the antibody with the antigenic PS1 peptide.