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Polarized distribution of PS1 after neuronal differentiation. Rat hippocampal neurons were cultured for 10 d and then double labeled for PS1 using Ab R28 to the PS1 C-terminal loop (A) and tau using Ab tau-1 (B). Arrows show co-localization of PS1 and tau in axons. Double labeling for PS1 (C) and MAP-2 (D) using Ab AP20 shows that PS1 is predominantly localized to the somatodendritic compartment (arrows) in differentiated neurons. Scale bars, 10 μm.
