Fig. 5.
Western blot analysis of the expression of NCAM, MBP, and MAG by cultured OLs. OLs were maintained for 2 d in culture on PLL (lanes 1), PLL plus TN-R 160 (lanes 2, 4, 5), and PLL plus TN-R 180 (lanes 3), as described above. Lanes 4, 5, Fab′ fragments of polyclonal antibodies to TN-R (50 μg/ml,lane 4) or preimmune rabbit IgG (100 μg/ml, lane 5) were added to the cultures 2 hr after plating. Protein extracts (from 1 × 106cells/lane) were subjected to SDS-PAGE and Western blot analysis by using polyclonal antibodies to NCAM and MAG or monoclonal antibody to MBP. Antibody binding was visualized with HRP-conjugated secondary antibodies and the ECL method for detection (Amersham), according to the manufacturer’s instructions. The apparent molecular weights (in kDa) are shown at the left margin.