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Explant cultures were treated with 150 μm DRB or 4 μm actinomycin D (Act D) for 0, 8, 16, or 40 hr and then processed for ISHH. For each anatomical region (A, AH;B, MH; and C,CH), TH mRNA levels per cell (O.D./cell) were measured, and the median value of each treatment group was plotted to calculate TH mRNA turnover.
